巻号一覧
5 巻 (1988)
- 3 号 p. 173-
- 2 号 p. 93-
- 1 号 p. 1-
5 巻, 2 号
選択された号の論文の9件中1～9を表示しています
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Munehisa KOGATA, Masahide KOBAYASHI, Manabu YAMAMURA, Koshiro HIOKI, M ...1988 年 5 巻 2 号 p. 93-101
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーThe selenium levels in the serum, malignant tissue, and normal tissue samples of gastrointestinal cancer patients as well as the daily urinary selenium output were measured in this study. Serum selenium levels of cancer patients were significantly lower than those of the control groups which consisted of surgical patients without cancer, age-matched non-hospitalized patients, and healthy young volunteers. Daily urinary outputs of selenium were not significantly different, however, between cancer patients and surgical patients without cancer. In patients with malignant diseases, the mean selenium concentration was higher in malignant tissues than in the normal adjacent tissues. Tissue selenium concentrations were reduced significantly in patients with malignant diseases compared with patients with benign ones. Serum selenium levels were lower in patients with regional or distant involvement; however, there was no correlation between tissue selenium concentration and the clinical stage of the tumor.抄録全体を表示PDF形式でダウンロード (853K) -
Akira TAMURA, Keiji KANDA, Tatsuzo FUJII, Yutaka HARANO, Mariko HARADA ...1988 年 5 巻 2 号 p. 103-109
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーBiochemical and morphological investigations were made on erythrocytes from two patients homozygous for genetic hypobetalipoproteinemia. Increased ratios of cholesterol/total phospholipids and sphingomyelin/phosphatidylcholine, and decreased membrane lipid fluidity were detected in the patients' cells as compared with those from a normal adult. Typical acanthocytes were observed which comprised 10-15% of each cell population of the patients. Such acanthocytosis was not corrected by incubation of the cells with either normal plasma, purified LDL, or phosphatidylcholine liposomes, but was corrected by treatment with phospholipase C which caused stomatocytosis of the cells. Incubation of the patients' cells with echinocytogenic agents such as flufenamic acid and lysophosphatidylcholine yielded more intensive echinocytosis than occurred with normal cells. Incubation of the former with a stomatocytogenic reagent, chlorpromazine, yielded weaker stages of stomatocytes than in the case of the latter.
These results suggest that the acanthocyte shape may not simply result from the abnormal lipid contents of the cell membrane, and that not only the observed acanthocytes are abnormal but also the other normal-looking cells of the patients may have some abnormalities in their membrane structure.抄録全体を表示PDF形式でダウンロード (3089K) -
Yoshiki MIYACHI, Sadao IMAMURA, Yukie NIWA1988 年 5 巻 2 号 p. 111-116
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーIn order to elucidate the mechanisms of anti-inflammatory activity of a new synthetic vitamin A-like compound (polyprenoic acid derivative, E-5166), the anti-oxidant effect of the agent was evaluated by examining the levels of reactive oxygen species both in the zymosan-stimulated neutrophil system and in the cell-free, xanthine-xanthine oxidase system. E-5166 inhibited the generation of hydroxyl radicals but had little effect on superoxide, hydrogen peroxide, or chemiluminescence when used at therapeutic concentrations. The favorable effects of E-5166 on several inflammatory skin disorders are discussed in connection with the anti-oxidant activity of the drug.抄録全体を表示PDF形式でダウンロード (521K) -
Yasuyuki SASAGURI, Minoru MORIMATSU, Ryuji NAKANO, Osamu TOKUNAGA, Kun ...1988 年 5 巻 2 号 p. 117-126
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーThe effects of linoleic acid hydroperoxide on macrophages derived from peripheral monocytes were investigated. The phagocytotic activity of macrophages was not inhibited in the presence of linoleic acid hydroperoxide up to 5nmol/ml (measured by the thiobarbituric acid reaction and expressed in terms of malondialdehyde), at which concentration cultured endothelial cells from the aorta are markedly injured. When we examined macrophages incubated with 5nmol/ml of the hydroperoxide by electron microscopy, however, the number of organelles was found to be increased and the electron density of the mitochondrial matrix was slightly decreased. As judged from the morphological changes, the susceptibility of macrophages to linoleic acid hydroperoxide seems to be in the same order as that of cultured smooth muscle cells from the aorta, but lower than that of cultured endothelial cells. In the presence of 15nmol/ml of the hydroperoxide, macrophages showed the features of marked degeneration, while still retained phagocytotic activity to a considerable extent. The significance of these phenomena in atherogenesis was discussed.抄録全体を表示PDF形式でダウンロード (9106K) -
Jun OKUDA, Kenji TOKUI1988 年 5 巻 2 号 p. 127-134
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーFe2+ and Fe3+ bound to protein in human serum were released by addition of TCA-HCl mixture after elimination of coexisting ascorbic acid, uric acid, etc. by centrifugation with an ultrafiltration membrane cone filter or after decomposition of these reducing agents with ascorbate oxidase and uricase. Fe2+ in the supernatant of the TCA-HCl mixture was determined at 535nm by the bathophenanthroline method, and the Fe3+ in the supernatant was reduced to Fe2+ by addition of ascorbic acid and successively assayed as Fe2+ by the bathophenanthroline method. Changes in the amount of Fe2+ and Fe3+ bound to human serum protein over a 24-h period were then studied by using the present method. A peak of Fe3+ in human serum seemed to appear in the morning, while almost no peak was observed for Fe2+ (average concentration: 10μM). So, the ratio of Fe2+/Fe3+ bound to human serum protein varied over the range of 0.5-5.0 within a day. The normal range of total iron concentration in human serum was 12-28μM.抄録全体を表示PDF形式でダウンロード (697K) -
Ichizo SHINODA, Rie TAKEUCHI, Masayuki KUROBE, Shoei FURUKAWA, Kyozo H ...1988 年 5 巻 2 号 p. 135-144
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーA highly sensitive two-site enzyme immunoassay system for mouse β-nerve growth factor (β-NGF) was developed, based on the sandwiching of the antigen between anti-β-NGF IgG antibody coated on a polystyrene bead and anti-β-NGF Fab' antibody-linked peroxidase (horseradish peroxidase, EC 1.11.1.7). Its discriminatory sensitivity was as low as 1pg/ml. Using this enzyme immunoassay system, we examined the levels of β-NGF in various tissues, serum, and plasma of mice. Among these, the level of β-NGF in submaxillary gland was found to be extremely high, as has been reported previously. Also, low levels of β-NGF were detected in the other tissues, serum, and plasma. The level in spleen was found to be relatively high compared with the levels in all but the submaxillary gland. To examine the molecular nature of NGF in plasma, serum or plasma was applied to a column of Sephadex G-100. β-NGF-like immunoreactive substance(s) appeared over a range from the void volume to the position at which a protein having a molecular weight of about 13, 000 daltons elutes. A major peak was observed at an elution position corresponding to a molecular weight of approximately 21, 000. By reducing serum or plasma with dithiothreitol, the elution profile was altered remarkably and the broad peak with β-NGF-like immunoreactivity was converted into two fractions, one appearing in the void volume and the other at the position corresponding to β-NGF. These results indicate that a part of the β-NGF may circulate as a complex form with some macromolecule(s) through disulfide linkage(s) in mouse plasma.抄録全体を表示PDF形式でダウンロード (956K) -
Kunihisa IWAI, Shuichi KIMURA1988 年 5 巻 2 号 p. 145-153
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーWe have investigated the efficiency of pheophorbide-dimer (Pheo-dimer) localization into tumors and the effect of photodynamic therapy (PDT) using Pheo-dimer on mice bearing FM3A tumors as a part of our search for new photosensitizers. Pheo-dimer was synthesized by covalently joining two molecules of pyropheophorbide a (Pyro) with an arginine as a cross-link. The in vitro photodynamic inactivation of FM3A cells by Pheo-dimer was less than that by pheophorbide a (Pheo). The FM3A tumor took up Pheo-dimer gradually as well as Pheo, but the time required for localization of Pheo-dimer was faster. The time for attaining the maximum concentration of Pheo in the tumor after injection was 18-24h, while that of Pheo-dimer was 9-12h. A small amount of Pheo-dimer was degraded to monomers by breakage of its cross-link in the body.
When irradiation was performed at 10h following administration of Pheo-dimer, the longer survival of mice bearing FM3A tumors was found in comparison with that obtained when Pheo was used. The utility of Pheo-dimer was thus expected in PDT.抄録全体を表示PDF形式でダウンロード (818K) -
Hironobu ISHIYAMA, Katsuya YAMASAKI, Takashi IMAIZUMI, Toshimi KANBE, ...1988 年 5 巻 2 号 p. 155-163
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーA subcutaneous dose of diethyldithiocarbamate (DDC) combined with a topical application of hydrochloric acid produced deep ulcers in the lesser curvature of the antrum in pylorus-ligated rats. The ulcers penetrated the tunica muscularis and formed an ulcer with an incidence of 100%. A significant decrease in Cu, Zn-superoxide dismutase (SOD) activity and a significant increase in the level of thiobarbituric acid (TBA) reactants in the antral mucosa occurred prior to the ulcer formation. Treatment with SOD and allopurinol significantly inhibited antral ulceration. These results suggest that superoxide radicals are involved in the pathogenesis of the ulcer and that this ulcer model is useful for studying gastric ulcer etiology.抄録全体を表示PDF形式でダウンロード (1969K) -
Kazuko HIRAI, Masami FUJIKI, Masako MATSUSHITA, Yoshimi OHNO1988 年 5 巻 2 号 p. 165-171
発行日: 1988年
公開日: 2010/02/25
ジャーナル フリーThe effects of dietary cholesterol on the relationship among cholesterol and fatty acid levels in the serum, liver, and heart of female rats were studied. When various levels of cholesterol (0, 0.25, 0.50, 0.75, and 1.00%) were added to a 10% butter diet with cholic acid, proportional increases appeared in liver weight (r=0.94), but no significant increase occurred in body or heart weight. An increase in dietary cholesterol induced increases in serum, liver, and heart cholesterol (r=0.72, r=0.87, and r=0.84, respectively). A direct relationship was found between the cholesterol levels for the serum and the liver (r=0.67) and for the serum and the heart (r=0.83). The ratio of polyunsaturated fatty acids/cholesterol decreased with cholesterol levels in serum, liver, and heart (r=-0.83, r=-0.74, and r=-0.77, respectively). The changes in heart cholesterol correlated positively with the changes in heart levels of palmitoleic acid (r=0.61), and the value of palmitoleic acid in the heart correlated with the values for serum palmitoleic acid (r=0.60). These results indicate that dietary cholesterol has profound effects on the levels of cholesterol and palmitoleic acid in the heart as in the serum and liver and show that changes in the amounts of cholesterol and palmitoleic acid in serum reflect changes in these amounts in the liver and heart.抄録全体を表示PDF形式でダウンロード (603K)
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