NGS データ解析環境の導入と新規遺伝子候補の探索

宮澤 由妃; 深野 義人; 見越 大樹; 井上 寿男; 丸岡 秀一郎; 黒田 和道; 権 寧博; 橋本 修; 山岸 賢司

doi:10.2477/jccj.2014-0058

Letters (Selected Paper)

Establishment of Next-Generation Sequencer Data Analysis Methods and Exploration of Novel Gene Candidates

Yuki MIYAZAWA, Yoshihito FUKANO, Taiju MIKOSHI, Toshio INOUE, Syuichirou MARUOKA, Kazumichi KURODA, Yasuhiro GON, Shu HASHIMOTO, Kenji YAMAGISHI

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Yuki MIYAZAWA
Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, 1 Nakagawara, Tokusada, Tamura, Koriyama, 963-8642, Japan
Yoshihito FUKANO
Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, 1 Nakagawara, Tokusada, Tamura, Koriyama, 963-8642, Japan
Taiju MIKOSHI
Department of Computer Science, College of Engineering, Nihon University, 1 Nakagawara, Tokusada, Tamura, Koriyama, 963-8642, Japan
Toshio INOUE
Department of Internal Medicine, Nihon University School of Medicine, 30-1, Oyaguchikamicho, Itabashi, 173-8610, Japan
Syuichirou MARUOKA
Department of Internal Medicine, Nihon University School of Medicine, 30-1, Oyaguchikamicho, Itabashi, 173-8610, Japan
Kazumichi KURODA
Department of Pathology and Microbiology Nihon University School of Medicine, 30-1, Oyaguchikamicho, Itabashi, 173-8610, Japan
Yasuhiro GON
Department of Internal Medicine, Nihon University School of Medicine, 30-1, Oyaguchikamicho, Itabashi, 173-8610, Japan
Shu HASHIMOTO
Department of Internal Medicine, Nihon University School of Medicine, 30-1, Oyaguchikamicho, Itabashi, 173-8610, Japan
Kenji YAMAGISHI
Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, 1 Nakagawara, Tokusada, Tamura, Koriyama, 963-8642, Japan

Keywords: NGS, Next Generation Seqencer, Novel gene, RNA-seq, Respiratory disease

JOURNAL FREE ACCESS

2014 Volume 13 Issue 6 Pages 310-311

DOI https://doi.org/10.2477/jccj.2014-0058

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Abstract

Next-generation sequencers (NGS) have made it possible to analyze entire genome sequence. NGS can be used for the identification of genes associated with a wide variety of diseases. We investigate RNAs encoded by intergenic regions to identify novel genes that are involved in respiratory diseases. The respiratory tracts of mice were exposed to lipopolysaccharide to establish a model of respiratory disease. RNA expression levels in the mouse exosome were analyzed by NGS to identify genes involved in the development of respiratory diseases. Several disease-related regions exhibited altered expression levels of intergenic RNAs.

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