2015 年 57 巻 1 号 p. 59-65
Structural analysis including hydrogen position is important for estimating the reaction mechanism of hydrolases. We applied neutron and X-ray structural analysis to a mysterious β-1,4 glucanase from a fungi Phanerochaete chrysosporium (PcCel45A). The joint analysis of neutron and X-ray structures showed that PcCel45A utilizing an imidic acid form of asparagine residue as a general base instead of typical acidic residues.