特集 電子線で何が観測できるか
水和したままのタンパク質や細胞を観る
－クライオ電子顕微鏡法の発展－
2019 年 61 巻 1 号 p. 43-50
詳細
2019 年 61 巻 1 号 p. 43-50
The electron cryo-microscopy is one of the most powerful techniques to elucidate biological molecular structure under nearly physiological condition. By single particle analysis, many three-dimensional structures of proteins and their complexes have been resolved at atomic resolutions. Furthermore, in situ molecular behaviours in cells and tissues have also been reported at nano-meter or sub-nanometer resolutions by electron tomography. I would here review current situations of electron cryo-microscopy and the principles of their analysis and so show its possibility.
