KEKの共同利用型クライオ電子顕微鏡の利用方法

抄録

In March 2018, KEK obtained a 200 kV cryogenic electron microscopy（cryo-EM; a Talos Arctica G2 with a Falcon 3EC direct electron detector）and prepared a pipeline for solving high-resolution structures of macromolecular biomolecules, such as proteins, DNA, and RNA. In October 2018, the cryo-EM facility in KEK opened to academic and industrial users. Our mission is three-fold：to provide cryo-EM machine time for external users, to support grid-prep and data collection（and single particle analysis, if needed）, and to help users acquire cryo-EM skills. One remarkable result is the single-particle analysis of an 860-kDa enzyme at 2.05 angstrom resolution, which suggested that we had established a proper protocol for cryo-EM operation and single-particle analysis. We will continue to support external users and carry out cryo-EM single particle analysis of macromolecular biomolecules.