抄録
The effects of protein loading on the enhancement of phosphatase activities were examined for four soil samples obtained from Kamo River, which runs through Takehara city in the Hiroshima prefecture. The results show that a marked enhancement of the phosphatase activities was observed in soils from St.3 and St.4, which are located in the estuarine water region. On the other hand, the soils from St.1∼2, in the upper or middle stream regions of the river showed a poor induction of the activities.
In addition to the analysis of the phosphatase activities, the protease activities and the hydrolytic products of the protein loaded in soils from the Kamo River were studied using SDS-PAGE (Sodium dodecyl sulfate-polyacryl amide gel electrophoresis). The results indicated that the soils from the middle reach (St.2) and estuarine water region (St.3∼4) have high protease activities. Moreover, the polypeptide patterns produced by the protease digestion in the soils were very similar between St.3 and St.4, which showed a marked enhancement of the phosphatase activities by protein loading, suggesting the presence of similar populations of microorganisms in the two regions.
