Cloning, nucleotide sequencing, and expression of the β-galactosidase-encoding gene (lacA) from Aspergillus oryzae

Yoshiyuki Ito; Takashi Sasaki; Katsuhiko Kitamoto; Chieko Kumagai; Kohjiro Takahashi; Katsuya Gomi; Gakuzo Tamura

doi:10.2323/jgam.48.135

Full Papers

Cloning, nucleotide sequencing, and expression of the β-galactosidase-encoding gene (lacA) from Aspergillus oryzae

Yoshiyuki Ito, Takashi Sasaki, Katsuhiko Kitamoto, Chieko Kumagai, Kohjiro Takahashi, Katsuya Gomi, Gakuzo Tamura

著者情報

キーワード: Aspergillus oryzae, β-galactosidase, gene cloning, genomic gene, lacA

ジャーナルフリー

2002 年 48 巻 3 号 p. 135-142

DOI https://doi.org/10.2323/jgam.48.135

詳細

抄録

lacA coding for β-galactosidase (β-gal) was cloned from the genomic DNA of Aspergillus oryzae RIB40. There were 9 exons in lacA and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. A. oryzae lacA was highly homologous to fungal β-gals, with the highest aa identity of 70.7% to A. niger lacA, and also showed significant identity to acid β-gals belonging to family 35 glycosyl hydrolases. Approximately 10 copies of lacA under control of A. oryzae glaA promoter were integrated into the chromosome of A. oryzae M-2-3. The recombinant strain expressed more than 700-fold of the β-gal activity as compared to the wild type strain under induction by maltose.

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