The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
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Nonomuraea maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere mud
Ismet AraTakuji KudoAtsuko MatsumotoYoko TakahashiSatoshi Omura
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2007 Volume 53 Issue 3 Pages 159-166


A strain of Nonomuraea was isolated from Maheshkhali, Cox's Bazar, an unexplored region of Bangladesh. Strain 16-5-14T is a Gram-positive, aerobic, non-motile actinomycete that formed branched substrate and aerial mycelia. On the basis of 16S rRNA gene sequence similarity studies, strain 16-5-14T was shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea kuesteri. Chemotaxonomic data supported allocation of the strain as a member of the genus Nonomuraea. The strain 16-5-14T contained MK-9(H4) as the major menaquinone, the polar lipid was phosphatidylethanolamine and major cellular fatty acids were observed as C16 : 0 (15.5%), iso-C16 : 0 (13.8%) and 10-methyl C17 : 0 (9.6%). Results of DNA-DNA hybridization and physiological tests allowed genotypic and phenotypic differentiation of strain 16-5-14T from closely related species N. kuesteri. Thus 16-5-14T represents a novel species of the genus Nonomuraea. On the basis of evaluation of the morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, Nonomuraea maheshkhaliensis sp. nov. (type strain, 16-5-14T=JCM 13929T=MTCC 8545T) is proposed.

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© 2007 by The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
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