抄録
Aspergillus niger grown on a synthetic medium containing sulfate as source of sulfur produced appreciably no protease, but if the sulfate was limmited in the medium, it produced protease actively irrespective of its poor growth. The optimum concentration of sulfate for the protease formation was 3×10-4M, whereas sulfate concentration supporting heavy growth of the fungus was 10-3M. By contrast, if the fungus was cultured in a complex medium containing peptone, the fungus produced protease actively irrespective of the high content of sulfur compounds.
The formations of proteases in the sulfur-deficient synthetic medium and in the complex medium seemed to depend on different physiological activities of the hyphae, but these enzymes produced in the two media showed similar chromatographic pattern, i.e., at least two proteases were produced in both cases, although the relative amount of these proteases were different in both cases. The mode of formation of these different proteases by growing hyphae was discussed.
