The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
ON THE DIFFERENCE IN ADSORPTION ON SEPHADEX GEL OF THE DEXTRANSUCRASE OF STREPTOCOCCUS BOVIS GROWN ON SUCROSE AND GLUCOSE MEDIA
TOSHIRO HAYASHIRYO IOROINAOHIRO OHARAMICHIO KOZAKI
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1988 Volume 34 Issue 3 Pages 213-219

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Abstract

The dextransucrase produced by orange-colored Streptococcus bovis No. 148 isolated from bovine rumen was investigated. The enzyme was produced in both sucrose and glucose media. The enzyme prepared from the glucose medium was specifically adsorbed on various Sephadex gels. But that from the sucrose medium was not adsorbed. When the enzyme from the glucose medium was incubated with a certain dextran, it was no longer adsorbed on Sephadex. There was no saccharide in the enzyme produced in glucose medium.

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