Abstract
This experiment was carried out in order to study what sort of fats as well as to what extent are hydrolyzed by the intestinal lipase. In addition, it was made in order to know how the activity of lipase is affected by adding bile acids. The substrates employed were tri-n-butyrin and triolein and they were hydrolyzed by the homogenates of mouse intestine.
Hydrolytic activity was calculated from the part of the substrates which was not hydrolyzed and was determined by the hydroxamic acid method. The effect of the bile acids added to the media was also studied. As a result, it was shown that tri-n-butyrin was readily hydrolyzed by the intestinal lipase and that this enzyme action was accelerated by adding a certain amount of bile acids. It was also found that triolein was hydrolyzed by the intestinal lipase, although not as readily as the case with tri-n-butyrin. The hydrolytic activity with triolein, however, was markedly accelerated in the presence of bile acids.
