Effects of Some Food Additives of Protein Biosynthesis by Rat Liver Slices and Cell-free Systems (Part 1)

Abstract

Effects of food additives on protein biosynthesis and on oxygen uptake of rat liver slices were examined by means of the incorporation of ¹⁴C-leucine into the total proteins. The following results were obtained.
1) Sodium dehydroacetate stimulated slightly ¹⁴C-leucine incorporation at the concentration of 5 mM, and inhibited its incorporation at 50 mM, but had little effect on oxygen uptake.
2) Sodium lauryl benzene sulfonate inhibited ¹⁴C-leucine incorporation by about 50% at 2.5 mM, and inhibited markedly the oxygen uptake by liver slices.
3) Potassium sorbate inhibited markedly ¹⁴C-leucine incorporation at the concentration higher than 50 mM, but had little effect on the oxygen uptake.
4) 2, 3-Acrylic amide inhibited ¹⁴C-leucine incorporation by about 30% at 0.005 mM, but had little effect on the oxygen uptake.
From these results, these additives inhibited specifically the protein biosynthesis without the effect on respiration except for Sodium lauryl benzene sulfonate which also inhibited the oxygen uptake.