Abstract
In order to obtain some information on the accumulation of 5'-nucleotide in the fruit body of Lentinus edodes (Shii-take in Japanese), the distribution of RNA and enzymatic activity were investigated as well as their changes during the growth of the fruit body.
1) Water soluble protein and RNA, which were richer in cap part than in stalk part of Shii-take, decreased during the growth. During the second half of growth period, these concentrations in cap part were in the following order : gill>upper surface>middle layer.
2) Phosphatase activity in cap part of Shii-take gradually decreased during the growth, but was larger at each growth step than in stalk part, where the activity kept constant during the growth. The distribution of phosphatase in cap part was somewhat localized to upper surface.
3) Nuclease activity in cap part was smaller at the first half of growth period and larger at the second half, than in stalk part. The distribution of nuclease in cap part was localized to gill, especially to the part of basidium and basidiospore.
