Studies on the Antigenicity of a Higher Molecular Fraction in α-Lactalbumin Digested with Pepsin
1992 Volume 43 Issue 5 Pages 383-388
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1992 Volume 43 Issue 5 Pages 383-388
The objective of this experiment was to examine the antigenicities of higher molecular fraction. (HMF) formed from α-lactalbumin. (α-La) digested with pepsin. The antigenicity was determined by comparison with the reactivities of anti HMF serum and anti α-La serum with peptides obtained by α-La treated with cyanogen bromide.
The peptides obtained by the severance at the position of methionine residue and the reduction of disulfide bonds in the primary structure of α-La strongly reacted with anti HMF serum, but the reactivities of anti α-La serum with their peptides were low. Further, it was suggested that a part of antigenic determinant of HMF existed in peptide 1-90 and 91-123 from α-La. These results indicate that the antigenic dereminants of HMF depend upon primary structure of its molecule.
