Characterization of Enterobacter cloacae GAO Isolated from Soaking Water of Sliced Apple

Abstract

The Enterobacter cloacae GAO arisen from plant and E. cloacae IAM 12349, Escherichia coli JAM 12119 arisen from intestine can be judged by simple methods.
The growth of Euterobacter cloacae GAO (abbreviated hereafter as GAO) was stimulated two and three times in optical density by addition of apple juice and casamino acid, respectively.
Cellobiose and xylose were utilized best as carbon sources by GAO, although the latter was slightly utilized by E. cloacae IAM 12349 (abbreviated hereafter as IAM 12349).
GAO grew in a wide range of pH 4-10, but E. coli IAM 12119 (abbreviated hereafter as E. coli) failed below pH 7 and above pH 10.
GAO proved positive in esculin hydrolysis test, while both IAM 12349 and E. coli proved negative. Their difference was clearly observed.