Salivary Gland Adenoid Cystic Carcinoma is Promoted by Increased POFUT1 Expression

抄録

Previous studies have reported the up-regulated expression of protein O-fucosyltransferase 1 (POFUT1) in several human malignant tumors. This study purposes to explore the relevance of POFUT1 expression to the clinical and biological characteristics of salivary gland adenoid cystic carcinoma (SACC). 54 clinical SACC samples were applied in immunohistochemistry to evaluate the practical expression of POFUT1. The cells from human submandibular gland (HSG), and the cell lines from salivary gland adenoid cystic carcinoma (SACC-83, SACC-LM) were applied in quantitative PCR and Western blots to confirm the elevated POFUT1 expression in SACC. The shRNA of POFUT1 were delivered into SACC lines to knock down POFUT1 expression, and then, the capacities of progressing, proliferating, colony forming and surviving in the SACC lines were evaluated. The clinical samples from SACC patients were found exhibiting a noticeably robuster POFUT1 expression compared to the noncancerous samples. Statistical analysis indicated a significant correlation of POFUT1 expression to the T classification of SACC. Similarly, the POFUT1 expression in SACC lines was also elevated compared to the normal salivary gland. The decreased POFUT1 expression in SACC lines remarkably suppressed cell proliferation, colony amount and survival. Therefore, the elevated POFUT1 expression enhances the proliferation and survival of SACC, which implicates that POFUT1 could be used as an index for SACC prognosis and a potential target for future therapy.