抄録
The TMIG (Tokyo Metropolitan Institute of Gerontology)-2DPAGE has been improved to achieve efficient proteome data exchange among collaborative research community. We developed an integrated XML viewer and the XML database system (PHP + PostgreSQL) for 2DPAGE. And we further optimized our TMIG-XML format for data sharing in the user community. The format is designed with 2 layers of the two-dimensional electrophoresis gel information and spot information in the gel images. It has the locale information of the spots. The clickable imagemap functions for the representation of the spot information are both available in the XML database system with PHP and in the viewer with Object Pascal (Delphi 7.0). Individual spots on an experimental gel image are easily matched to corresponding spots on our standard gel image of our TMIG-2DPAGE proteome database using the viewer. The viewer must be the efficient collaborative tool in the user community. The viewer and the database system both allow the user to click on the spots on the gel image to obtain the appropriate entry in the major sequence databases. Moreover the TMIG-2DPAGE database client and the private TMIG-2DPAGE in the TMIG's intranet as the simple laboratory information management system will be constructed. The additional format with the project information, the worker information and time-stamp of major items will be imported to the expanded TMIG-XML format.
