Abstract
We saccharified steam-exploded eucalyptus with various cellulase concentrations for 0-18 h. After saccharification, the weight of insoluble substrate was measured and the change of the cellulase concentration in liquor was investigated using Bradford method. The saccharification efficiency reached about 90% with 300- and 600- fold diluted cellulase and the added cellulase was adsorbed quickly onto the insoluble substrate. The cellulase concentration in liquor turned to increase at 2 h and 6 h for 300- and 600-fold diluted cellulase, respectively. On the other hands, it remained almost constant with 1200- and 2400- fold diluted cellulase. It seemed that cellulase concentration started to increase after the saccharification efficiency reached around 50 %, indicating that the rate of cellulase detachment is related to the substrate composition during enzymatic saccharification. Lower cellulase loading led to less cellulase adsorption onto the final residue.
