We focused on aerobic fermentation of biomass as a method to supply CO2 to plant cultivation facilities. In addition, acetoin and 2,3-Butanediol (2,3-BDO) produced by fermentation were reported to have growth-promoting effects on plants. In this study, fermentation experiments of oyster mushroom waste were performed using Acremonium cellulase and Bacillus subtilis in a fed-batch reactor with aeration. Effects of fermentation conditions such as moisture and substrate mass on production of Acetoin and 2,3-BDO were investigated. The results showed that acetoin and 2,3-BDO were produced when the oxygen concentration decreased to the microaerobic level.
