抄録
Most whole cell biocatalysts produce various byproducts by the activity of host metabolic enzymes, and their bioprocesses have low yields. The metabolic pathways have to be deleted by the methodology of metabolic engineering and fermentation in order to increase the yields and productivities. However, these processes consume time and effort for the construction of efficient bio-processes. We propose the fully-synthesized biocatalysts for efficient bioproduction by using a psychrophile Shewanella livinstonensis Ac10. The biocatalysts have the synthetic pathway for desired chemicals by the heterologous expression of genes from the mesophiles in a psychrophilic organism. The cultured cells were treated with heat at 40-50℃ in order to inactivate psychrophilic metabolism and increase the permeability of substrate into the cells. The fully-synthesized biocatalysts have significant advantages in productivity (high yields and without byproducts) and operativity (simple handling without enzyme extraction) because all the substrates can be used for the productions of desired chemicals by simple heat treatment of biocatalysts. We applied the fully-synthesized biocatalysts for efficient production of 3-hydroxypropionaldehyde and 1,3-propandiol from glycerol. The concentration of 3-HPA was 200 mM in maximum with about 100% conversion rate in 1 hour.
