Abstract
D2O concentration was measured spectrophotometrically in an amount as small as 1μl of haemolymph in the female yellow peach moth by incorporating a newly designed capillary cell to the CRESPI and KATZ's method. A proportional relationship between the calling time (φr) and the D2O concentration was observed under a 22°C and 15L-9D regime, the scotophase of which was supplied by different energy levels of red light. The higher the red light intensity, the longer the delay of φr per 10% D2O concentration i.e. 20.7min at ca. 1μW/cm2, 21.4min at ca. 10μW/cm2 and 27.7min at ca. 60μW/cm2. From these results, it was concluded that there was an interaction between the D2O concentration in the haemolymph and the light intensity of the scotophase on the calling rhythm of the species.
