Validation study on a method for multiresidue analysis of pesticides in vegetables and fruits with supercritical fluid extraction and LC-MS/MS

Abstract

Supercritical fluid extraction (SFE) was applied to extraction of pesticides from vegetables and fruits. Residues were extracted from homogenized samples mixed with celite and water-absorbent polymer with supercritical carbon dioxide. Measurement was performed by LC-MS/MS. With the Japanese method validation guideline as a reference, the method was assessed in 5 agricultural products spiked with 114 pesticides at 0.01 and 0.05 µg/g. Compounds at each level were extracted from 2 samples on 5 separate days. The trueness of the method for 58–78 pesticides in each samples was 70–120%, and the repeatability and within-run reproducibility were also consistent with the guideline. This method is available as a multiresidue analysis method for vegetables and fruits.