2021 Volume 28 Issue 2 Pages 90-97
To check the authenticity of the food labeling regarding genetically modified organisms (GMOs), we need to detect recombinant DNA remaining in processed foods. In this study, we investigated on the detection of corn starch-derived recombinant DNA in beer. We made beer samples from identity preserved corn starch (low GMO content) and non-identity preserved corn starch (high GMO content). Recombinant DNA was detected only from start material, and not detected from the other intermediates and final products irrespective of GMO contents in starch. Real-time PCR analysis detecting 18S rDNA and internal positive control proved that the impossible detection of recombinant DNA was caused not by PCR inhibitors but by extremely small DNA amounts in beer.