The analytical method was validated by HPLC to inspect residual clopidol in game meats. Clopidol was extracted with acetonitrile/ methanol mixture and McIlvaine buffer. The impurities in the extract were removed by n-hexane partition and a solid-phase column, InertSep PLS-2. After removing the solvent, the extract was resolved in a 40% acetonitrile solution. Clopidol in the purified solution was quantitatively determined using HPLC with a photodiode array detector (PDA). An interfering peak was not observed in the chromatograms of game meat extracts. The limit of quantification of this method was 0.01 µg/g in game meats. Recovery tests performed method validation at the maximum residue limit concentration to evaluate the trueness, repeatability (RSDr), and with-in laboratory reproducibility (RSDwr). The validation results exhibited sufficient trueness (88% and 93%) and precision (RSDr ≦ 6% and RSDwr ≦ 9%). These values fulfilled the criteria of the validation guideline for residual pesticide analysis in Japan. The time required to prepare eight test sample solutions was less than five hours. This method can help inspect residual clopidol in game meats.