抄録
The analytical method for the residual oxytetracycline in processed Japanese apricot beverages was validated. The residual OTC was extracted using a McIlvaine buffer containing EDTA-2Na. The solid-phase column, InertSep PLS-2, was employed to remove the matrix from the extract. Following the removal of the elution solvent from the solid-phase column, the extract was dissolved in a potassium phosphate solution. The concentration of OTC in the test solution was determined quantitatively using HPLC with a photodiode array detector (PDA). The chromatograms of the blank test solutions exhibited no measurement interfering peaks. Recovery tests were conducted to validate the analytical method at 0.2 μg/g to evaluate the trueness, repeatability (RSDr), and within-laboratory reproducibility (RSDwr). The validation results for Japanese apricot juice and liqueur exhibited sufficient trueness (90% and 75%) and precision (RSDr ≤ 7% and RSDwr ≤ 8%). The simulated Japanese apricot liqueur revealed that ethanol in beverages reduced the recovery rate of OTC. These results may assist in the inspection of residual OTC in processed Japanese apricot beverages.
