厚生労働省通知アフラトキシン試験法の複数機関による評価研究

抄録

The newly notified analytical method for aflatoxins was established by improving the previous reports. An inter-laboratory validation studies were conducted to validate the analytical method. Aflatoxins B₁, G₁, G₂ and B₂ spiked into peanuts, pistachio nuts, corn, red pepper and nutmeg at the level of 10 ng/g were analyzed in replicates in 5 laboratories. Mean recoveries of aflatoxin B₁ were 77.9〜92.7% and those of aflatoxins G₁, G₂ and B₂ were 81.5〜92.5%, 84.7〜82.2% and 83.7〜89.1%, respectively. Repeatability relative standard deviations of aflatoxins B₁, G₁, G₂ and B₂ in five crops were in the ranges of 2.9〜7.3%, 2.1〜6.5%, 1.3〜5.2% and 1.9〜4.6%, respectively. Reproducibility relative standard deviations of aflatoxins B₁, G₁, G₂ and B₂ in five crops were in the ranges of 6.5〜10.1%, 2.5〜14.2%, 6.5〜10.1% and 5.3〜10.6%, respectively. These results suggested that the proposed method is reliable and reproducible for the inspection of aflatoxin levels in peanuts, pistachio nuts, corn, red pepper and nutmeg.