自家新鮮海綿骨および同種脱脂凍結乾燥海綿骨の筋膜下移植における新鮮遊離骨膜・脱脂凍結乾燥骨膜の被包効果に関する実験的研究

抄録

The following experiments were designed to study the influence of periosteal wrapping of a cancellous bone graft.
The experiments were divided into 6 groups according to whether the cancellous bone graft was fresh or defat-lyophilized, with or without periosteal wrapping, and to autologous or defat-lyophilized periosteum. Each material was transplanted underneath the fascia lata in adult dogs and grafted material was observed with histological and histomorphometric methods.
The results were as follows:
1. Fresh autologous cancellous bone grafts without periosteal wrapping induced active bone formation after 1 week, but by 4 weeks, new bone formation at the surface of the graft was almost arrested and partial absorption of transplanted and/or newly-formed bone by surrounding tissues was recognized.
2. Defat-lyophilized cancellous bone homografts did not induce new bone formation, and absorption was observed at the graft margin by encroachment of the surrounding tissue.
3. In fresh autogenous cancellous bone wrapped with fresh periosteum, new bone formation was delayed in the early phase because of retarded revascularization as compared with that which occurred without periosteal wrapping. However, after 4 weeks, the quantity of newly-formed and transplanted bone trabeculae, as well as the amount of newly-formed bone, was much more than that observed without periosteal wrapping.
4. In defat-lyophilized cancellous bone homograft, no difference was noted between the presence and absence of the wrapping with fresh periosteum autograft.
5. Wrapping with homologous defat-lyophilized periosteum hindered new bone formation, in both bone autograft and homograft.
The fresh periosteum autograft could support new bone formation and also protect the graft from encroachment by the graft bed in the fresh bone autograft. In contrast, fresh periosteum showed no activity in the defat-lyophilized bone homograft. These results suggest that fresh periosteal wrapping to fresh cancellous bone autograft may facilitate the bone inductive activity, and it is also suggested that these transplantations can be dominated not only by the condition of the periosteum but also by the state of the recipient site.