扁平上皮癌細胞に対するlymphokine-activated killer (LAK) 細胞の接着性, 細胞傷害性におよぼすinterferon-γ (IFN-γ) およびtumor necrosis factor (TNF-α) の影響についての研究

腫瘍細胞上の細胞接着分子の関与について

抄録

We investigated the effects of interferon-γ(IFN-γ) and tumor necrosis factor (TNF-α) treatment on NR-S 1 cells (murine squamous cell carcinoma). Since NR-S 1 cells treated with a combination of IFN-γand TNF-αincreased susceptibility to conjugation and lysis by lymphokine activated killer (LAK) cells, we examined the participation of celladhesion molecules on the NR-S 1 cells in this phenomenon. Untreated NR-S 1 cells expressed MHC class I, MHC class II, intercellular-adhesion molecule-1 (ICAM-1), and ICAM-2. Treatment with IFN-γinduced the expression of MHC class I and ICAM-1 molecules, but had no effect on the expression of MHC class II and ICAM-2 molecules on NRS 1 cells. Treatment with TNF-αinduced the expression of ICAM-1 molecules, but had no effect on the expression of MHC class I, MHC class II and ICAM-2 molecules. However, treatment with a combination of IFN-γand TNF-αstrongly induced expression of MHC class I and ICAM-1 molecules and slightly induced expression of ICAM-2 molecules, as compared with IFN-γor TNF-αalone.
The results indicate that the expression of ICAM-1 molecules, makes NR-S 1 cells more adhesive to LAK cells and more susceptible to lysis by LAK cells.