A Semi-Quantitative PCR Method for the Detection of Low Levels of Apoptotic DNA Fragmentation in a Heart Failure Model

Craig S. McLachlan; Jian Lin Yin; Catherine Driussi; Patricia R. Jusuf; Brett Hambly; Mark A. McGuire

doi:10.2170/jjphysiol.50.281

Short Communications

A Semi-Quantitative PCR Method for the Detection of Low Levels of Apoptotic DNA Fragmentation in a Heart Failure Model

Craig S. McLachlan, Jian Lin Yin, Catherine Driussi, Patricia R. Jusuf, Brett Hambly, Mark A. McGuire

著者情報

キーワード: apoptosis, PCR ladder, heart failure

ジャーナルフリー

2000 年 50 巻 2 号 p. 281-284

DOI https://doi.org/10.2170/jjphysiol.50.281

詳細

抄録

Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.

責任著者(Corresponding author)

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