イネいもち病感染初期の連続観察のための剥離葉鞘接種法
1983 年 49 巻 2 号 p. 256-258
詳細
1983 年 49 巻 2 号 p. 256-258
We deviced a sliced-sheath inoculation method for continuous observation of leaf sheath cells infected with Pyricularia oryzae Cavara. By this method, the aspects of intracellular hyphal growth in the same tissues were observed successively without sectioning of tissues at various times after inoculation. The growth of infection hyphae in sliced-sheath cells was less than that in non-sliced-sheath cells at 32-40hr after inoculation. It might be due to the physiological changes of sheath cells resulted by slicing tissues. The method proposed here seems to be suitable for study of rice-Pyricularia oryzae interaction.
