抄録
Two cases of herpes vaginitis (42 and 36 yearold housewives) were diagnosed by cytologic examination. The cytologic, histopathologic and electron microscopic studies were made of patient materials, and infected HEL cells.
Three cases of herpes zoster associated with malignant tumors (two cases of uterine cancer-59 and 51 year-old housewives, and a case of reticulum cell sarcoma-49 year-old female) were studied by same methods.
The alteration was compared with infected cells of HSV-II and herpes Zoster.
Result as follows: -1: The virus was isolated from vaginal materials in case 2, and was identified as HSV-II with the use of HF and UW-268 streins.
2: Variable changes were found in the squamous and columnal cells of cervical epithelium. The early visible structural alteration showed glassylike transluent mononuclear cells with swelling of the cytoplasm and the nucleus. Characteristic diagnostic feature is lobulated and multinucleated giant cells with intranuclear inclusion bodies.
3: The cultured HEL cells were examined by cytologic and electron microscopic observation at 48, 72 and 96 hrs. after viral infection. The alteration of infected cells revealed similar features for human materials. The immature viral particles (100mμ) with central cores or empty capsid in nucleus, and enveloped mature particles (200 mμ) in cytoplasmic vacuoles were found in the infected HEL cells.
4: The materials of herpes zoster were examined by useing vesicular fluid and epidermis. The infected squamous cells revealed enlarged lobular and a few multinuclear changes without glassylike swollen. Electron microscopically, viral particles appeared to have similar structures as HSV-II in infected HEL cells.
