Detection of HPV infection by DNA-RNA hybridization and cytological findings

Abstract

We detected cases of HPV-DNA positive in cervical dysplasia and cervical cancer by using the RNA probe on human papilloma virus (HPV).
1) Cases diagnosed by cytological analysis that were also HPV-DNA positive: There were 2 (50%) of 4 cases in class Ina, 2 (50%) of 4 cases in class Bib, 1 (50%) of 2 cases in class IV, and 2 (33%) of 6 cases in class V. Therefore 7 (44%) of 16 cases in a class more advanced than class Ma, were positive.
2) Cases diagnosed by pathological analysis that were also HPV-DNA positive: There was 1 (100%) case of mild dysplasia, 3 (43%) of 7 cases of CIS, and 2 (29%) of 7 cases of SCC.
3) 3 (43%) of 7 cases of HPV-DNA positive displayed koilocytes.
4) 85 (99%) of 86 cases detected by mass-screening of uterine cancer were HPV-DNA negative, and were of class I and class II. Only one case was HPV-DNA positive, and was proved to be in class IIIa.