From April 1964 to May 1967, the cytologic examination of urine and washing specimens of the bladder with saline solution of α-chymotrypsin of 261 patients in the National Cancer Center Hospital was made. All materials was processed according to the methods of May-Giemsa (Pappenheim) and of Papanicolaou after centrifugation.
1. In 261 cases, 88 were surely proved to be the cases of carcinoma of the urinary tract histologic ally and clinically; in these 88 cases, 21 were ex amined only after some treatments. The remaining 67 cases include 6 carcinomas of the kidney, 55 ca ncers of the renal pelvis, ureter, bladder and uret hra, and 4 carcinomas of the prostate.
2. The accuracy of cytodiagnosis of the 67 cases with the cancer of urinary tract was 68. 7 per cent and of the 49 cases of bladder was 77. 6 per cent.
3. The stickiness of cells to the glass slide is one of the most important factors for the accuracy of the cytologic diagnosis of urinary tract. The meth ods of the fixation after drying for the May-Gie-msa and of the spray fixation with mixture of polyethylene-glycol and isopropyl alcohol for the Papanicolaou are very efficient for the accurate diagnosis.
4. The accurate diagnosis of the carcinoma of bladder was also increased by using washings of the bladder and urine specimens.
5. The relationship of the histological grade (Ash, Ackerman) and the cytologic features was studied in 17 cases of the transitional cell rcarcin oma of bladder.