Abstract
The present paper deals with the quantitative analysis of tomato fruit pigments which may be influenced by environmental and cultural factors, especially with procedures of the measurement. Two varieties, such as Matsudo Ponderosa and Kurihara were used.
(1) In analizing pigments the column chromato-graphic method was used, in which calcium carbonate and active alminum oxide were used as adsorbents and aceton, petroleum benzin and ethyl ether as solvents.
(2) In determining the the concentrations of pigments in the extracts, the extinction coefficients were measured for chlorophyll, xanthophyll, carotene and lycopene with wave length 660, 440, 448 and 470mμ respectively, by using the 1cm cell of BECKMANN DU spectrophotometer. The concentrations of each pigment were estimated from extinction coefficients obtained by using standard calibrat ed curve.
(3) Total pigments in Matsudo Ponderosa showed much higher contents than those in Kurihara.
