Root Induction from Pear Floral Organ Callus I

Abstract

Effects of the addition of 2, 4-D and kinetin, each or both, to the Linsmair and Skoog medium on the callus induction from floral organs of Pyrus serotina culta REHDA cv. Nijisseiki and the root or shoot differentiation from the induced callus tissues were investigated.
Although callus tissues were induced from sepals, stamens and petals of buds collected before anthesis on every medium with or without 2, 4-D or kinetin, only the callus induced with 2, 4-D grew well on every medium, especially on 2, 4-D media in the subculture.
Root formation was recognized in the callus subcultured on the medium containing 2, 4-D at 0.1ppm and also in the callus transferred from the 2, 4-D medium onto the one containing adenine at 20ppm. Shoot formation from callus, however, did not occur on any kinds of media prepared in this experiment.