Abstract
Excised tulip bulb scale segments were able to generate adventitious buds when cultured on a modified Murashige-Skoog medium fortified with auxin and kinetin. Transferring the buds to a medium low in auxin and kinetin induced further development. Bulb formation occured at the proximal end of buds receiving a chilling treatment at 5°C, but failed to occur in unchilled buds. The optimal duration of the chilling period was found to be about 80-100 days. Bulb formation was enhanced by the application of NAA together with kinetin at low concentrations, although at high concentrations formation was delayed. Sucrose was also necessary for bulb formation, and its optimum concentration was found to be range between 4 and 6%. Culture temperature for the bulb formation was optimal at 25°C.
