Journal of the Japanese Society for Horticultural Science
Online ISSN : 1880-358X
Print ISSN : 0013-7626
ISSN-L : 0013-7626
Polymorphism of Chloroplast DNA in Citrus
Masashi YamamotoShozo Kobayashi
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1996 Volume 65 Issue 2 Pages 291-296

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Abstract

Chloroplast DNA (cpDNA) diversity was studied in Citrus, Poncirus and Fortunella, based on restriction fragment length polymorphism (RFLP). Total genomic DNA was digested with restriction endonucleases singly or in combination, Southern blotted, and hybridized with a Nicotiana tabacum cpDNA probe. According to the fragment patterns, Citrus was divided into four groups; mandarin/citron, pummelo, Yuzu, and lime. The fragment patterns of mandarin/citron group (mandarin, citron and tangor) differed from those of pummelo group (pummelo, sweet orange, sour orange, lemon, ' grapefruit, Kunenbo, Hassaku, Natsudaidai, Sudachi, Hyuganatsu and Iyo) in Pst I, Pst I/Pvu II, Pst I/Kpn I and Pst I/ Sac I digestion. The fragment patterns of lime were identical to pummelo group in Pst I and Pst I/ Sac I digestion, but differed in Pst I/Pvu II and Pst I/Kpn I digestion. Yuzu was not distinguishable from mandarin/citron group in the fragment patterns of Pst I, Pst I/ Pvu II and Pst I/ Kpn I digestion, but was distinguishable in that of Pst I/ Sac I digestion. The fragment patterns of Poncirus and Fortunella were always identical to those of mandarin/citron group.

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