Abstract
AFLP analysis, using FITC (fluorescein isothiocyanate) and biotin detection systems, was applied to molecular genetics to obtain the information on reproducibility, efficiency and frequency of polymorphisms, using the peach as the experimental subject. An almost identical band pattern was obtained from different methods of DNA extraction and between replications. FITC detection with DNA sequencer made AFLP analysis more accurate and efficient and biotin detection simplified the cloning of the target bands. AFLP analysis produced 2.5 polymorphic bands per primer between 'Akame' and Juseitou', which were almost 20 times more than those obtained by RAPD analysis. Thirty eight AFLPs were obtained out of 334 amplified fragments among 8 cultivars by 8 primer combinations, which could discriminate even closely related cultivars. In conclusion, we confirmed that AFLP analysis could provide useful genetic markers to execute the cultivar identification, the parentage, and mapping work in peach.
