RAPD Analysis of 'Koshu' Grape and Its Self-Pollinated Progenies

Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to a Japanese grape cultivar 'Koshu' and nine selected self-pollinated progenies. The RAPD analysis, using 18 primers, resulted in identical banding patterns with one primer but different banding patterns with the other primers. A total of 108 bands, including three novel bands that were not found in the patterns of the parent, were detected. Percentage similarity between each pair of vines ranged from 68.4% to 90.8%. The similarity values among progenies were comparatively low, whereas those between progenies and parent were comparatively high. Therefore, the data indicate that diversity among the nine vines of the self-pollinated seedlings was as broad as between pre-existing cultivars.