Abstract
Human plasma fraction derivatives, Plasmanate (5% plasma protein solution) and globulin (15%), were made from 2 kinds of human plasma pools proven either positive or negative of Au-antigen by immunoelectrosynelesis technic (IES). Both plasma pools and their products were quantitatively measured for Au-antigen and -antibody by Solid Phase Radioimmunoassay.
While Au-antigen positive plasma manifested 104 of Au-antigen concentration, derived Plasmanate and ISG preparations showed the titer of 103 and 101 respectively. While Au-antigen negative plasma manifested 101 of Au-antigen, derived Plasmanate and ISG showed the titer smaller than 10°.
There was no difference in the antibody-binding ability of Au-antigen present in Plasmanate (Au-antigen positive origin) before and after heat-treatment at 60°C for 10 hours. Au-antibody was not found in Plasmanate. Au-antibody in the globulin preparation from Au-antigen negative plasma was found higher than that from Au-antigen positive plasma.
