Abstract
Biochemical and serological characteristics were investigated on the anti-A antibody in the serum from a group B male and on the anti-B antibody in the serum from a group A male who were immunized with A and B soluble specific blood group substances.
1) The sera were titrated for anti-A and anti-B agglutinins by the saline and the bromelin methods before and after 2-mercaptoethanol treatment. Changes in the titers were traced for three months.
2) The sera were fractionated with DEAE cellulose. The IgG anti-B activity showed at peak at about the 15th day followed by a slow uphill.
The IgA anti-B increased markedly from about the 40th to the 76th day.
The IgM anti-B antibody titer decreased after the initial peak at about the 15th day. IgG anti-A titer increased slowly to about the 46th day and became stable thereafter. IgA anti-A revealed a slow peak at about the 46th day, nearly diminishing at about the 82nd day. IgM anti-A activity reached a plateau at about the 15th day and began to fall from about the 46th day.
