Biochemical and serological characteristics were investigated on the anti-A antibody in the serum from a group B male and on the anti-B antibody in the serum from a group A male who were immunized with A and B soluble specific blood group substances.
1) The sera were titrated for anti-A and anti-B agglutinins by the saline and the bromelin methods before and after 2-mercaptoethanol treatment. Changes in the titers were traced for three months.
2) The sera were fractionated with DEAE cellulose. The IgG anti-B activity showed at peak at about the 15th day followed by a slow uphill.
The IgA anti-B increased markedly from about the 40th to the 76th day.
The IgM anti-B antibody titer decreased after the initial peak at about the 15th day. IgG anti-A titer increased slowly to about the 46th day and became stable thereafter. IgA anti-A revealed a slow peak at about the 46th day, nearly diminishing at about the 82nd day. IgM anti-A activity reached a plateau at about the 15th day and began to fall from about the 46th day.

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Leukocyte antibodies in maternal sera were investigated with latex particle agglutination.
1) 60 cases from 190 maternal sera showed abnormal titer higher than 1:8. Distribution of leukocyte antibody titers from groups O, A, B and AB maternal sera showed no difference.
2) There was no difference in the frequencies of the detected leukocyte antibodies between the maternal sera from A B O or Rh compatible matings and those from incompatible ones.
3) Leukocyte antibodies in maternal sera with different number of pregnancies showed no significantly different distributions.
4) Mothers possessing high leukocyte antibodies showed higher rate of stillbirths. Relation of leukocyte antibodies with spontaneous abortions in early pregnancy was not observed.
5) A case of leukopenic newborn baby due to feto-maternal immunity was reported, suggesting that the baby's leukocytes were affected by the mother's antibody produced from leukocyte group incompatibility.

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