骨髄単核細胞からの lymphokine-activated killer (LAK) 細胞の誘導とそのキラー活性について

抄録

Bone marrow mononuclear cells (BM) were obtained from allogeneic bone marrow donors. The BM-LAK were generated by culturing of the BM in the medium containing recombinant interleukin-2 for several days. The natural killer (NK) activity of BM against K562 was very low. However, the killer activity of BM-LAK against Raji was very high, and significantly higher than that of the peripheral blood lymphocyte (PBL)-LAK. The killer activity of PBL-LAK decreased shortly after 2 weeks of the culture, whereas the BM-LAK maintained high killer activity for a long period. The BM-LAK demonstrated relatively high percentage of CD4^-CD8^-CD16⁺, CD8⁺CD16⁺ and CD8⁺CD11b⁺ phenotypes, compared to the BM. It is likely that these phenotypes represent active killer cells. BM contained many adherent cells which were monocytes and macrophages. It is necessary to remove these adherent cells from the culture within one week, because these cells produced the suppressive factors against the proliferation of BM-LAK. The high killer activity of BM-LAK is effective for adoptive immunotherapy, and may be useful on the purging of tumor cells in autologous bone marrow transplantation.