MTSゲル遠心法による感染症検査の有用性

抄録

To evaluate the gel centrifugation method for infectious disease tests, basic experiments were performed with various commercial kits by the hemo- or particle-agglutination method. Optimal conditions for the method were considered to be an incubation time of 15min, an incubation temperature of 24-26°C (room temperature), and a half sensitized-particle concentration.
The gel method was compared with conventional microplate methods for the utility of infectious disease tests with the patients' specimens. There were no discrepancies in the detection tests of HBs-antigen and HCV-, CMV-, HIV, and HTLV-I-antibodies between the gel method and the microplate method, although the gel system detecting HCV-antibody titers was 25% less sensitive than the microplate method. Three of the 83 specimens showed discrepancy in the HBs-antibody detection. They were weak positive by the gel method but negative by the microplate method. We recognized that the test by the gel centrifugation method was reliable, reproducible, and speedy to obtain the results. The gel method could be used as a routine and emergency test for infectious diseases.