Studies on the Pseudomonas aeruginosa Report III

抄録

1) Two mutant strains obtained from a strain of Pseudomonas aeruginosa isolated from the blood of a septic patient; another strain of Ps. aeruginosa is isolated from an ulcerated lesion of the same patient.
Of these strains, endotoxins are obtained on a trichloracetic acid method.
2) Purified endotoxins are electrophoretically homogenous.
The solutions of 0.1 per cent of these endotoxins are Molisch reaction, Bial's orcin-HC1 reaction, Ninhydrin test and Sakaguchis' test positive.
3) Polysaccharide fractions of No.134 and No.002 endotoxin consist of glucose, xylose, rhamnose and unknown monosaccharide. Polysaccharide fraction of No.117 endotoxin consists of glucose, galactose, xylose, rhamnose and glucosamine.
4) The endotoxin of No.117 strain shows no typical pyrogenic effect on rabbits and its toxicity to mouse is much lower than that of ordinary Ps. aeruginosa strain.
I wish to express my great indebtedness to Prof. S. Osawa for his assistance in the reading of proof and offering many useful suggetions. The kindness of Associate Prof. E. Inoue is also gratefully acknowledged. Thanks, deeply felt, are due to Associate Prof. S. Higashi, Kyoto University who has contributed to the experiment.