緑膿菌に関する研究

特に第2螢光部の産生に関するアミノ酸の効果について

抄録

I. Observations were made on the growth and pigment production of eight strains of Ps. aeruginosa on the synthetic media, each of which contained one of the thirty three different kinds of amino acid and its delivatives. The results were as follows:
1) In the media which containedα-and β-alanine, glutamic acid, γ-amino-n-butyric acid, praline, all the test strains showed good growth and clear pigmentation, and especially the one which contained γ-amino-n-butyric acid revealed a pronounced effects.
2) In the media which contained asparagine, aspartic acid, glutamine, arginine, ornithine, glutaric acid, α-ketoglutaric acid and histidine, all the test strains showed a good growth, but less pigment and fluorescence production.
3) In the media which contained valine, leucine, isoleucine and tyrosine, some of the test strains showed a good growth with a moderate pigmentation.
4) In the media which contained glycine, serine, threonine, phenylalanine, α-and β-amino-n-butyric acids and citrulline, all the test strains grown poorly showing very small colonies after one week cultivation.
5) In the media which contained norleucine, cystine, cysteine and methionine, none of the test strains could grow.
II. Four strains (No.001,002,117 and 58) were cultivated on the synthetic liquid media, each of which contained one of the twelve kinds of amino acid, which allowed these four strains a good growth. After one week cultivation, the gel filtration for every culture filtrate were performed, collecting and analyzing the Fluorescence II fractions. The results were as follows.
1) Whenever all the strains tested showed good growth, Fluorescence II fraction was obtained regardless with the production of visible colour and Fluorescence I fraction.
2) Paper electrophoretic analysis of Fluorescence II fraction showed that the strain No.58 was able to produce the fluorescent substances, which were characteristic of Ps. aeruginosa, from almost all the amino acids tested.
3) The paper electrophoretic pattern of Fluorescence II of pyocyanin strain No.002was very similar to that of No.58 but melanin strains No.001 and 117 were quite different with that of No.58.