Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome

Keigo Kinoshita; Toshikatsu Shinka; Youichi Sato; Hiroki Kurahashi; Hiroe Kowa; Gang Chen; Mayumi Umeno; Kazunori Toida; Emi Kiyokage; Takuro Nakano; Susumu Ito; Yutaka Nakahori

doi:10.2152/jmi.53.117

Originals

Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome

Keigo Kinoshita, Toshikatsu Shinka, Youichi Sato, Hiroki Kurahashi, Hiroe Kowa, Gang Chen, Mayumi Umeno, Kazunori Toida, Emi Kiyokage, Takuro Nakano, Susumu Ito, Yutaka Nakahori

著者情報

Keigo Kinoshita
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School
Toshikatsu Shinka
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Youichi Sato
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Hiroki Kurahashi
Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University
Hiroe Kowa
Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University
Gang Chen
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Mayumi Umeno
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Kazunori Toida
Department of Anatomy and Cell Biology, The University of Tokushima Graduate School
Emi Kiyokage
Department of Anatomy and Cell Biology, The University of Tokushima Graduate School
Takuro Nakano
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School
Susumu Ito
Department of Digestive and Cardiovascular Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School
Yutaka Nakahori
Department of Human Genetics and Public Health, Institute of Health Biosciences, The University of Tokushima, Graduate School

キーワード: HSFY, mHSFYL, HSF, Y chromosome, spermatogenesis

ジャーナルフリー

2006 年 53 巻 1,2 号 p. 117-122

DOI https://doi.org/10.2152/jmi.53.117

詳細

抄録

Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence [mHSFYL (Riken cDNA 4933413G11Rik)], which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis. J. Med. Invest. 53: 117-122, February, 2006

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）