卵巣灌流法の手技と成績

抄録

The rabbit ovary were perfused after ligation of branches of the ovarian artery and cannulation of this artery. perfusion appratus consisted of a pulsatile rotary pump, an oxygenator and a chamber with circulating medium. Tissue culture medium 199 was supplemented with insulin, heparin and antibiotics, and oxgenated by gas mixture of 95% O₂ and 5% CO₂. Follicle rupture, maturation of oocytes and progesterone production of the perfused ovary were observed after administration of hCG or other agents into the medium. Although maturation of oocytes and synthesis of progesterone of the perfused ovary were shown at the same rate as the in situ ovary, follicle rupture occurred earlier. prostaglandin, histamine and norepinephrine did not cause maturation of oocytes nor steroid production but made follicles rupture. No sequence of production of histamine and prostaglandin or interrelationship in their activity could be detected in the ovulatory process.