ましんウイルス持続感染Vero細胞治癒実験とウイルスの性状

抄録

Characteristics of persistently infected Vero cell lines, R-5-PI-Vero and 448-PI-Vero, established in our laboratory were studied. A persistently infected cell line, 448-PI-Vero was completely cured with 0.3% human γ-globulin in culture medium, and the cured cell culture did not yield infective virus. In the cured cells, neither the virus specific antigen nor a virus specific protein, presumably nucleocapsid protein, was detected with the imrnunofluorescent technique and SDS-PAGE, respectively.
Homologous interference phenomenon, which had been shown in the 448-PI-Vero, was not observed in the cured cell culture. These findings suggest that our persistently infected cell culture is a carrier culture.
Virus replication in 448-PI-Vero decreased at 39.5C, but recovered when the incubation temperature was shifted to 35C. Temperature-sensitivity, in terms of EOP (39.5C) / (35C), has decreased in virus from 448-PI-Vero, as compared to the original P-448 virus which was a temperature-sensitive mutant.
Replication of virus in 448-PI-Vero was not inhibited with 1 μg/ml actinomycin D, but inhibited with 1 ii. μg/ml cycloheximide with a lag of 6 hours.
Thermostability of virus from R-5-PI-Vero and 448-PI-Vero did not considerably differ from the original R-5 and P-448 virus, respectivel.