抄録
Non-frozen murine bone marrow preserved at various temperatures was tested for viability of the committed and pluripotent stem cells. Each stem cell examined retained its proliferative capacity most actively when stored at 4°C in α-medium containing 15% fetal calf serum. Under these experimental conditions, the recovery of the colony-forming ability of CFU-C and CFU-M was 50% and 74% respectively after storage for four days, while it was 26% and 14% respectively for CFU-E and BFU-E after storage for the same period of time. The proliferative capacity of the pluripotent stem cells which was prerequisite for successful bone marrow transplantation was preserved at satisfactory levels; the mean recovery on the 4th day of storage at 4°C being 67% for CFU-S and 45% for CFU-Mix.
The result indicates that a conventional CFU-C assay offers an useful index for judging proliferative capacity of the pluripotent stem cells in non-frozen bone marrow stored for transplantation.
